Cyclin E1 – RMab

For In Vitro Diagnostic Use

Cyclin E1 forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition phase of the cell cycle that detremines cell division. The Cyclin E/CDK2 complex phosphorylates p27Kip1 (an inhibitor of Cyclin D), taggint it for degradation and thus promoting expression of Cyclin A, allowing progression to the S phase. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Apart from the function in cell cycle progression, cyclin E/CDK2 plays a role in the centrosome cycle by phosphorylating nucleophosmin (NPM). NPM is then released from binding to an unduplicated centrosome, thereby triggering duplication. Cyclin E/CDK2 has also been shown to regulate the apoptotic response to DNA damage via phosphorylation of FOXO1.
Overexpression of Cyclin E correlates with tumorigenesis. It is involved in various types of cancers, including breast, colon, bladder, skin, and lung cancer.

Antibody Type Rabbit Monoclonal
Clone EP126
Isotype IgG
Reactivity Paraffin, Frozen
Localization Nuclear
Control Placenta, Bladder, Colon, Breast Cancer, Ovarian Carcinoma

Cyclin E1 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.